##plugins.themes.bootstrap3.article.main##

Polymerase chain reaction which is the widely utilized method had made progressive imprint in scientific research has its solid focuses and inadequacies. It presents the chance of defilement through the spreading of amplicon mist concentrates in the laboratory environment. To help lessen the probability of tainting, good laboratory practice ought to be practiced consistently. This review is centers to setting up a PCR laboratory and techniques for counteraction and devastation of contamination, and quality control.

References

  1. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature. 1989;339(6221):237. doi:10.1038/339237a0.
     Google Scholar
  2. Aslanzadeh J. Preventing PCR amplification carryover contamination in a clinical laboratory. Ann Clin Lab Sci. 2004;34(4):389-396.
     Google Scholar
  3. Varanat M, Maggi RG, Linder KE, Horton S, Breitschwerdt EB. Cross-contamination in the molecular detection of Bartonella from paraffin-embedded tissues. Vet Pathol. 2009;46(5):940-944. doi:10.1354/vp.08-VP-0259-B-BC.
     Google Scholar
  4. Kalle E, Kubista M, Rensing C. Multi-template polymerase chain reaction. Biomol Detect Quantif. 2014;2: 11-29. doi:10.1016/j.bdq.2014.11.002.
     Google Scholar
  5. Lorenz TC. Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies. J Vis Exp JoVE. 2012;(63). doi:10.3791/3998.
     Google Scholar
  6. Guidelines for Environmental Infection Control in Health-Care Facilities: (545922006-001). Published online 2003. doi:10.1037/e545922006-001.
     Google Scholar
  7. Bio-Pharmaceutical Cleanroom Design Guidelines. Accessed June 10, 2019. https://www.portafab.com/bio-pharmaceutical-cleanroom-design.html.
     Google Scholar
  8. Mifflin T. Setting up a PCR laboratory. CSH Protoc. 2007;2007:pdb.top14. doi:10.1101/pdb.top14.
     Google Scholar
  9. Fabre A-L, Colotte M, Luis A, Tuffet S, Bonnet J. An efficient method for long-term room temperature storage of RNA. Eur J Hum Genet. 2014;22(3):379-385. doi:10.1038/ejhg.2013.145.
     Google Scholar
  10. January 7 P, Share 2014. Free Up Your Freezer with These Room-Temperature DNA Storage Solutions. Accessed June 11, 2019. http://www.biocompare.com/Editorial-Articles/152844-Free-Up-Your-Freezer-with-These-Room-Temperature-DNA-Storage-Solutions/.
     Google Scholar
  11. 7 Tips to Make PCR Primers Last Longer and PCR Reactions Run Better. GEN - Genetic Engineering and Biotechnology News. Published June 27, 2012. Accessed June 11, 2019. https://www.genengnews.com/magazine/7-tips-to-make-pcr-primers-last-longer-and-pcr-reactions-run-better/.
     Google Scholar
  12. PCR Troubleshooting | LSR | Bio-Rad. Accessed June 11, 2019. http://www.bio-rad.com/en-us/applications-technologies/pcr-troubleshooting?ID=LUSO3HC4S.
     Google Scholar
  13. Pushparaj PN. Revisiting the Micropipetting Techniques in Biomedical Sciences: A Fundamental Prerequisite in Good Laboratory Practice. Bioinformation. 2020;16(1):8-12. doi:10.6026/97320630016008.
     Google Scholar
  14. Standard_Operating_Procedures.pdf. Accessed June 11, 2019. https://www.icmr.nic.in/sites/default/files/guidelines/Standard_Operating_Procedures.pdf.
     Google Scholar