Research Article

Detecting and Analyzing Hydrolytic Enzymes of Industrial Significance in two Streptomyces Strains Isolated from the Soil

Olaitan Akintunde
University of Louisiana at Lafayette, USA
* Corresponding author
DOI icon 10.24018/ejbio.2021.2.3.214

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Submitted 2021-05-31
Published 2021-06-21

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Article Main Content

Two Streptomyces strain were isolated from a soil sample in Louisiana. They were identified via 16S rRNA sequencing and phylogeny. To detect the presence of hydrolytic enzymes, starch, carboxymethyl-cellulose (CMC), lipase reagent, and Milk (casein) were used as substrate to detect the production of amylase, cellulose, lipase and casease respectively. Both strains showed the ability to hydrolyze starch, and cellulose, while only strain SWHR10 displayed lipase activity. In addition, strain SWHR10 showed better amylase and cellulase activity. Hemolysis, gelatinase and catalase tests were also conducted. This study further validates that Streptomyces remain a powerhouse of hydrolytic enzymes with industrial and economic importance.

Keywords: Hydrolytic enzymes, cellulase, biotechnology, Lipase, Streptomyces

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