Antimicrobial Activity of Moringa oleifera Leaf Extracts against Streptococcus pneumoniae Isolates

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  •   Richard Kagia

  •   Carolyne Chepkirui

  •   Michael Walekhwa

  •   Teresa Ogeto

  •   Titus Suge

  •   Mary Murithi

  •   Ferdinand Ndubi

  •   Esbon Wambugu

  •   Hadan Baiwo

  •   Zablon Malago

  •   Fred Kipsang

Abstract

Pneumococcal diseases (PD) constitute a major threat to the global public health stability especially in the developing world. The risk is greater for children under 5 years, the elderly and persons living with compromised immune systems. Antibiotic agents, conjugate as well as polysaccharide vaccines have been available for several decades and have saved many lives. However, the burden of PD has consistently remained high resulting in poor quality of life and strained economic tenets. Constant resistance to available antibiotic agents and low access to vaccines constitute major setbacks to efforts meant to scale down the burden of PD. The need to explore more viable and promising options is not only urgent but also untenable. We profiled the pharmacological credentials of Moringa oleifera extracts as a possible efficacious alternative to conventional prophylactic and therapeutic interventions.  Methanol and ethanol extracts of air-dried leaves of Moringa oleifera plant were subjected to phytochemical screening to identify the presence of carbohydrates, proteins, saponins, alkaloids, tannins, flavonoids, phyto-sterols, oils, and fats. A working solution of the two extracts was prepared by dissolving 20 mg & 40 mg of the extracts in 1ml of 99.9% dimethyl sulfoxide solvent. Sterilized 6 mm blank discs were loaded with 20ul of the final extract concentrations and incubated at 45 ºC for 20 hours. Dimethyl sulfoxide (100% DMSO) impregnated discs and 30 mcg ceftriaxone antibiotics were used as negative and positive controls respectively. Colon morphology on gentamicin blood agar (GBA) and blood agar (BA), gram staining and optochin assay were leveraged to identify Streptococcus pneumoniae. Disk diffusion method on Muller Hinton agar was used to evaluate sensitivity of Streptococcus pneumoniae to the subject extracts. Methanol and ethanol extracts were found to contain carbohydrates, proteins, saponins, tannins, flavonoids, steroids, fixed oils, alkaloids, and cardiac glycosides. Both extracts were found to contain flavonoids, tannins, and phenolic compounds. Ethanol & methanol extracts had lower anti-bacterial activity as compared to the 30 mcg positive control. Further exploration to reveal the anti-bacterial activity of aqueous based extracts on Streptococcus pneumoniae is strongly recommended


Keywords: Moringa oleifera, Pneumococcal disease, Streptococcus pneumoniae

References

Jones, K. E., Patel, N. G., Levy, M. A., Storeygard, A., Balk, D., Gittleman, J. L., & Daszak, P., “Global trends in emerging infectious diseases,” Nature, 2008. 451(7181), 990–993. https://doi.org/10.1038/nature06536.

Feldman, C., & Anderson, R., “Recent advances in the epidemiology and prevention of Streptococcus pneumoniae infections,” F1000Research, 2020. https://doi.org/10.12688/f1000research.22341.1.

Sanchez, E. and J. L. B., “Pneumococcal Infections (Streptococcus pneumoniae),” Retrieved from: https://emedicine.medscape.com/article/225811-overview. 2020.

Ghrairi, T., Jaraud, S., Alves, A., Fleury, Y., El Salabi, A., & Chouchani, C., “New Insights into and Updates on Antimicrobial Agents from Natural Products,” BioMed. Research International. 2019, https://doi.org/10.1155/2019/7079864.

Kirubakari, B., Shanmugapriya, Sangeetha, T., Vijayarathna, S., Chen, Y., Kanwar, J. R., Sasidharan, S., “Antibacterial and antifungal agents of higher plants,” Natural Bio-active Compounds: Volume 1: Production and Applications 2019, pp. 493–508. https://doi.org/10.1007/978-981-13-7154-7_16.

Abdull Razis, A. F., Ibrahim, M. D., & Kntayya, S. B., “Health Benefits of Moringa oleifera,” Asian Pacific Journal of Cancer Prevention, 2014. 15(20), 8571–8576. https://doi.org/10.7314/APJCP.2014.15.20.8571.

Patel, K, P; M. M., Kanzariya, N. R., Vaghela, K. R., Patel, D. R. K., & Patel, D. N. J., “In-Vitro Hepatoprotective Activity of Moringa Oleifera Lam. Leave on Isolated Rat Hepatocytes,” 2010. https://www.researchgate.net/publication/286757389_In-vitro_hepatoprotective_activity_of_Moringa_oleifera_Lam_Leave_on_isolated_rat_hepatocytes.

Stohs, S. J., & Hartman, M. J., “Review of the Safety and Efficacy of Moringa oleifera,” Phytotherapy Research, 2015. 29(6), 796–804. https://doi.org/https://doi.org/10.1002/ptr.5325.

Mehta, A., & Agrawal, B., “Investigation into the mechanism of action of Moringa oleifera for its anti-asthmatic activity,” Advances in Traditional Medicine, 2008. 8(1), 24–31. https://doi.org/10.3742/OPEM.2008.8.1.024.

Tahir, K., Mugal, M. T., & Haq, I. U., “Moringa oleifera: a natural gift-A review,” 2010, 2(11), 775–781.

Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H., “Moringa oleifera: a food plant with multiple medicinal uses,” Phytotherapy Research, 2007, 21(1), 17–25. https://doi.org/https://doi.org/10.1002/ptr.2023.

Gopalakrishnan, L., Doriya, K., & Kumar, D. S., “Moringa oleifera: A review on nutritive importance and its medicinal application,” Food Science and Human Wellness, 2016. 5(2), 49–56. https://doi.org/10.1016/j.fshw.2016.04.001.

Fahey, J. W., Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1., 24. 2005.

Pari, L., & Kumar, A. N., “Hepatoprotective Activity of Moringa oleifera on Antitubercular Drug-Induced Liver Damage in Rats,” Journal of Medicinal Food, 2005. https://doi.org/http://doi.org/10.1089/10966200260398206.

Toppo, R., Roy, B. K., Gora, R. H., Baxla, S. L., & Kumar, P., “Hepatoprotective activity of Moringa oleifera against cadmium toxicity in rats,” Veterinary World, 2015, 8(4), 537–540. https://doi.org/10.14202/vetworld.2015.537-540.

Mera, I. F. G., Falconí, D. E. G., & Córdova, V. M., “Secondary metabolites in plants: Main classes, phytochemical analysis and pharmacological activities,” Revista Bionatura, 2019. https://doi.org/10.21931/RB/2019.04.04.11.

Gebregiorgis Amabye, T., & Mekonen Tadesse, F., “Phytochemical and Antibacterial Activity of Moringa Oleifera Available in the Market of Mekelle,” Journal of Analytical & Pharmaceutical Research, 2016, 2(1). https://doi.org/10.15406/japlr.2016.02.00011.

Khatib, M, S. Toxicological and Pharmacological Use of Anticancer Compounds. Springer, 2014 Vol. 3, pp. 227. https://books.google.co.ke/books?id=PetiDwAAQBAJ&pg=PA227&lpg=PA227&dq=Khatib,+2014+moringa&source=bl&ots=IwuTzQVCeE&sig=ACfU3U1CAXpR3iEIRKuiGHuedQkRIunM4Q&hl=en&sa=X&ved=2ahUKEwil5ePrgp3yAhULkRQKHbftDvgQ6AF6BAgXEAM#v=onepage&q=Khatib%2C%202014%20moringa&f=false

Chinedu, A., Ajibaye, O., Anyasor, G., Fakoya, O., & Okebugwu, O. Effect of Extraction Solvents on Phenolic, Flavonoid and Antioxidant Activities of Three Nigerian Medicinal Plants. Nature and Science, 2011 9(7). file:///C:/Users/Hp/Desktop/paperpub.pdf

Jorgensen, E, J., Deyang, X., Christoph, C., Heidi, A, E., David, R., Mohammed, S, M et al. Origin and evolution of transporter substrate specificity within the NPF family. E-life. 2017, https://doi.org/10.7554/eLife.19466.001

Torres-Castillo, J. A., Sinagawa-García, S. R., Martínez-Ávila, G. C. G., López-Flores, A. B., Sánchez-González, E. I., Aguirre-Arzola, V. E., Gutiérrez-Díez, A., “Moringa oleifera: Phytochemical detection, antioxidants, enzymes and antifugal properties,” Phyton, 2013, 82, 193–202.

Torres-Castillo, J. A., Sinagawa-García, S. R., Martínez-Ávila, G. C. G., López-Flores, A. B., Sánchez-González, E. I., Aguirre-Arzola, V. E., Gutiérrez-Díez, A., “Moringa oleifera: Phytochemical detection, antioxidants, enzymes and antifugal properties,” Phyton, 2013, 82, 193–202.

Nayak, G., Rao, A., Mullick, P., Mutalik, S., Kalthur, S. G., Adiga, S. K., & Kalthur, G., “Ethanolic extract of Moringa oleifera leaves alleviate cyclophosphamide-induced testicular toxicity by improving endocrine function and modulating cell specific gene expression in mouse testis,” Journal of Ethnopharmacology, 2020, 259. https://doi.org/10.1016/j.jep.2020.112922.

Makita, C., Chimuka, L., Steenkamp, P., Cukrowska, E., & Madala, E., “Comparative analyses of flavonoid content in Moringa oleifera and Moringa ovalifolia with the aid of UHPLC-qTOF-MS fingerprinting,” South African Journal of Botany, 2016, 105, 116–122. https://doi.org/10.1016/j.sajb.2015.12.007.

Ravindra, A. V., Priya, R., & Siddheshwar, S. A., “Pharmacological Review on Moringa Oleifera,” World Journal of Pharmaceutical Research, 2019, 88(8), 910–920.

Das, S. K., J., B. D., P.V., P., Das, S., Behera, S. P., Veilumuthu, P., & Christopher, J. G., “Investigation on the Phenolic Content in Moringa oleifera and Its Antimicrobial Activity,” Indian Journal of Agricultural Research, (Of). 2020. https://doi.org/10.18805/ijare.a-5636.

Elgamily, H., Moussa, A., Elboraey, A., El-Sayed, H., Al-Moghazy, M., & Abdalla, A., “Microbiological assessment of Moringa oleifera extracts and its incorporation in novel dental remedies against some oral pathogens,” Macedonian Journal of Medical Sciences, 2016, 4(4), 585–590. https://doi.org/10.3889/oamjms.2016.132.

Kumari, C., Virk, A. K., Kumari, S., Gupta, T., Rolta, R., Li, X., & Kulshrestha, S., “Identification of potential targets for Thymidylate Synthase and Amp-C βlactamase from of non-alkaloidal fractions of Moringa oleifera leaves,” Current Pharmaceutical Biotechnology, 2021, 22. https://doi.org/10.2174/138920102266621011.

Yee, M. M. A., “Comparative Studies on Antimicrobial Activity and Antioxidant Activity on Different Extracts of Leaf, Bark and Root of Moringa oleifera Lamk (Drumstick tree),” International Journal of Recent Innovations in Academic Research ISSN, 2019. 3(7), 24–34.

Enwa, F. O., Omojate, C. G., & Adonu, C. C., “A review on the phytochemical profile and the antibacterial susceptibility Pattern of some clinical isolates to the ethanolic leaves extract of Moringa oleifera LAM,” International Journal of Advanced Research, 2013, 1(2320), 226–238.

Kou, X., Li, B., Olayanju, J. B., Drake, J. M., & Chen, N., “Nutraceutical or pharmacological potential of Moringa oleifera Lam,” 2018. Nutrients. https://doi.org/10.3390/nu10030343.

Metwally, F. M., Rashad, H. M., Ahmed, H. H., Mahmoud, A. A., Abdol Raouf, E. R., & Abdalla, A. M., “Molecular mechanisms of the anti-obesity potential effect of Moringa oleifera in the experimental model,” Asian Pacific Journal of Tropical Biomedicine, 2017, 7(3), 214–221. https://doi.org/10.1016/j.apjtb.2016.12.007.

Okoro, S.O, Kawo, A. H. and A., “Phytochemical Screening, Antibacterial and Toxicological,” Bayero Journal of Pure and Applied Sciences, 2014, 7(1), 105–115.

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How to Cite
Kagia, R., Chepkirui, C., Walekhwa, M., Ogeto, T., Suge, T., Murithi, M., Ndubi, F., Wambugu, E., Baiwo, H., Malago, Z., & Kipsang, F. (2021). Antimicrobial Activity of Moringa oleifera Leaf Extracts against Streptococcus pneumoniae Isolates. European Journal of Biology and Biotechnology, 2(4), 28–32. https://doi.org/10.24018/ejbio.2021.2.4.223