Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review


  •   Eustache T. A. E. Agbadje

  •   Arnaud Agbidinoukoun

  •   Martine Zandjanakou-Tachin

  •   Gilles T. H. Cacaï

  •   Corneille Ahanhanzo


Bananas and plantains are among the most important food crops in Central and West Africa. Their plantation is lead to many problems. In the recent decades, biotechnology tools using in vitro culture technics are used for the mass and free disease plantlets production in order to increase the bananas production and the yield. The main way of in vitro tissue culture at this end is the direct organogenesis i.e., the ability of plant tissues to form various organs de novo by shoots or roots induction to differentiate from a cell or cell clusters. This review aims to summarize the main results obtained in the organogenesis of bananas and plantains (Musa spp.) under in vitro conditions and to identify the challenges during the process. The research articles used in this review show that micropropagation is a reliable alternative to conventional production system of bananas and plantains planting material. However, the use of the in vitro micropropagation for bananas and plantains entails choosing the optimal explant type and size according to objectives. Benzylaminopurine remains the preferred cytokinin for in vitro banana and plantain shoot proliferation, while the use of thidiazuron appears to be more and more common. Whichever cytokinin used, the optimal cytokinin concentration for shoot proliferation is genotype dependent. This review also focuses on the causes and control measures of the two major banana and plantain micropropagation constraints: lethal tissues browning/darkening and microbial contaminations. It showed that applying the suitable and available control measure, according to the evolution of culture, is necessary. All this available information on the in vitro conditions makes banana and plantain cultivars in vitro organogenesis possible.

Keywords: explant asepsis maintenance, growth regulators, lethal browning control, microbial contamination, Musa spp


Resmi, L., and Nair, A.S. (2007). Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India. Plant Cell Tissue Organ Cult. 2007; 88, 333-338.

Vuylsteke, D. (1998). Shoot-tip culture for the propagation, conservation and distribution of Musa germplasm (IITA) 1998.

Kishor, H., Abhijith, Y., and Manjunatha, N. (2017). Micropropagation of Native Cultivars of Banana-A Critical Review. Int. J. Pure App. Biosci. 2017; 5, 1559-1564.

Strosse, H., Van den Houwe, I., and Panis, B. (2004). Banana cell and tissue culture-review. In Banana improvement cellular, molecular biology and induced mutations, S.M. Jain, and R. Swennen, eds. (Science Publishers, Inc), 2004; pp. 1-12.

Teisson, C., and Côte, F.-X. (1997). Micropropagation of Musa species (bananas). In Biotechnology in Agriculture and Forestry 39 High-Tech and Micropropagation V, Y.P.S. Bajaj, ed. (Springer), 1997; pp. 103-126.

Sandoval, J., and Müller, L.E. (1987). Influencia del tamaño de explante en la propagación in vitro de cuatro cultivares de Musa. Turrialba (IICA) 1987;42, 243-248.

Youmbi, E., and Ngaha, D. (2004). Expression in vitro des capacités organogènes des bourgeons axillaires chez le bananier plantain (Musa spp.). Fruits 2004; 59, 241-248.

Youmbi, E., Tchananyambeu, M., and Fonbah, C. (2014). In vitro Proliferation Ability of Axillary Buds in Musa spp. Agron. Afr. 2014;26, 1-7.

Heller, R., Esnault, R., and Lance, C. (1995). Physiologie végétale. Tome 2. Développement (Masson) 1995.

Habiba, U., Reza, S., Saha, M.L., Khan, M., and Hadiuzzaman, S. (2002). Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention. Plant Tissue Cult. 2002; 12, 117-124.

Cronauer, S., and Krikorian, A. (1985). Aseptic multiplication of banana from excised floral apices. HortScience 1985; 20, 770-771.

Cronauer-Mitra, S.S., and Krikorian, A. (1988). Determinate floral buds of plantain (Musa AAB) as a site of adventitious shoot formation. Ann Bot. 1988; 61, 507-512.

Kumar, K.G., Krishna, V., Venkatesh, V., and Pradeep, K. (2011). High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale-AB Genome. Plant Tissue Cult. Biotech. 2011; 21, 199-205.

Darvari, F.M., Sariah, M., Puad, M., and Maziah, M. (2010). Micropropagation of some Malaysian banana and plantain (Musa spp.) cultivars using male flowers. Afr. J. Biotechnol. 2010; 9, 2360-2366.

Mahadev, S.R., Kathithachalam, A., and Marimuthu, M. (2011). An efficient protocol for large-scale plantlet production from male floral meristems of Musa spp. cultivars Virupakshi and Sirumalai. In vitro Cell. Dev. Biol.-Plant 2011; 47, 611-617.

Harirah, A.A., and Khalid, N. (2006). Direct regeneration and rapd assessment of male inflorescence derived plants of musa cuminata cv. berangan. AsPac J. Mol. Biol. Biotechnol. 2006; 14, 11-17.

Wirakarnain, S., Hossain, A., and Chandran, S. (2008). Plantlet production through development of competent multiple meristem cultures from male inflorescence of banana, Musa acuminta cv. “Pisang Mas” (AA). Am. J. Biochem. Biotechnol. 2008; 4, 325-328.

Youssef, M., James, A., Mayo-Mosqueda, A., Ku-Cauich, J.R., Grijalva-Arango, R., and Escobedo-GM, R.M. (2010). Influence of genotype and age of explant source on the capacity for somatic embryogenesis of two Cavendish banana cultivars (Musa acuminata Colla, AAA). Afr. J. Biotechnol. 2010; 9, 2216-2223.

Al-Amin, M., Karim, M., Amin, M., Rahman, S., and Mamun, A. (2009). In vitro micropropagation of banana. Bangladesh J. Agril. Res. 2009; 34, 645-659.

Shankar, C.S., Balaji, P., and Sekar, D.S. (2014). Mass Propagation of Banana (Musa spp.) cv. Grand Naine through Direct Organogenesis by Benzyl Adenine Purine and Kinetin. J. Acad. Ind.Res. 2014; 3, 92-97.

Lassois, L., Lepoivre, P., Swennen, R., van den Houwe, I., and Panis, B. (2013). Thermotherapy, chemotherapy, and meristem culture in banana. In Protocols for micropropagation of selected economically-important horticultural plants, M. Lambardi, E. A. Ozudogru, S. M. Jain, eds. (Springer), 2013; pp 419-433.

Waman, A., Bohra, P., Sathyanarayana, B., Umesha, K., Mukunda, G., Ashok, T., and Gowda, B. (2015). Optimization of factors affecting in vitro establishment, ex vitro rooting and hardening for commercial scale multiplication of silk banana (Musa AAB). Erwerbs-Obstbau 2015; 57, 153-164.

Banerjee, N.a.d.L.E. (1985). A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain). Plant Cell Rep. 4, 351-354.

Madhulatha, P., Anbalagan, M., Jayachandran, S., and Sakthivel, N. (2004). Influence of liquid pulse treatment with growth regulators on in vitro propagation of banana (Musa spp. AAA). Plant Cell Tissue Organ Cult. 2004; 76, 189-192.

Kone, T., Kone, M., Kone, D., Kouakou, T.H., Traore, S., and Kouadio, Y. (2010). Effet de la photopériode et des vitamines sur la micropropagation du bananier plantain (Musa AAB) à partir de rejets écailles de rang1. J. Appl. Biosci. 2010;26, 1675-1686.

Kalimuthu, K., Saravanakumar, M., and Senthilkumar, R. (2007). In vitro micropropagation of Musa sapientum L. (Cavendish Dwarf). Afr. J. Biotechnol. 6, 1106-1109.

Shiragi, M., Baque, M., and Nasiruddin, K. (2008). Eradication of Banana Bunchy Top Virus (BBTV) and Banana Mosaic Virus (BMV) from Infected Plant of Banana cv. Amritasagar through Meristem Culture. South Pac. Stud. 29, 17-41.

Gandonou, G., Ahanhanzo, C., Agbangla, C., Agbidinoukoun, A., Doussoh, A., Cacai, G., and Dossoukpevi, R. (2012). Micropropagation in vitro de la variété locale «Aloga» du bananier plantain (Musa x paradisiaca L.) au Bénin. Int. J. Biol. Chem. Sci. 2012; 6, 1102-1111.

Kahia, J., Ndaruhutse, F., Waweru, B., Bonaventure, N., Mutaganda, A., Sallah, P.Y., Kariuki, N.P., and Asiimwe, T. (2015). In vitro propagation of two elite cooking banana cultivars-FHIA 17 and INJAGI. Int. J. Biotechnol. Mol. Biol. Res. 2015; 6, 40-47.

Farzinebrahimi, R., Rashid, K., Taha, R.M., and Yaacob, J.S. (2013). Effective Sterilization Protocol for Micropropagation of Musa coccinea (Musa Spp). In International Conference on Agriculture and Biotechnology (IACSIT Press, Singapore) 2013.

Jarret, R., Fisher, J., and Litz, R. (1985). Organ formation in Musa tissue cultures. J. Plant Physiol. 1985; 121, 123-130.

Hamill, S., Sharrock, S., and Smith, M. (1993). Comparison of decontamination methods used in initiation of banana tissue cultures from field-collected suckers. Plant Cell Tissue Organ Cult. 1993; 33, 343-346.

Jafari, N., Othman, R.Y., and Khalid, N. (2011). Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan. Afr. J. Biotechnol. 2011; 10, 2446-2450.

Dixon, R.A., and Gonzales, R.A. (1994). Plant cell culture: a practical approach (IRL press) 1994.

Davies, P.J. (2010). The plant hormones: their nature, occurrence, and functions. In Plant hormones (Springer) 2010; pp. 1-15.

Spíchal, L. (2012). Cytokinins–recent news and views of evolutionally old molecules. Funct. Plant. Biol. 39, 267-284.

Gana, A. (2011). The role of synthetic growth hormones in crop multiplication and improvement. Afr. J. Biotechnol. 2011; 10, 10330-10334.

Ganapathi, T., Mohan, J., Suprasanna, P., Bapat, V., and Rao, P. (1995). A low-cost strategy for in vitro propagation of banana. Curr. Sci. 1995; 68, 646-650.

Mok, M.C., Martin, R.C., Dobrev, P.I., Vanková, R., Ho, P.S., Yonekura-Sakakibara, K., Sakakibara, H., and Mok, D.W. (2005). Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition. Plant Physiol. 2005; 137, 1057-1066.

Mok, M., Mok, D., Armstrong, D., Shudo, K., Isogai, Y., and Okamoto, T. (1982). Cytokinin activity of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron). Phytochemistry 1982; 21, 1509-1511.

Shirani, S., Mahdavi, F., and Maziah, M. (2009). Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoottips. Afr. J. Biotechnol. 2009; 8, 5755-5761.

Cronauer, S.S., and Krikorian, A. (1984). Multiplication of Musa from excised stem tips. Ann Bot. 1984; 53, 321-328.

Buah, J. (2014). Suitability of Cassava Starch as a Gelling Agent for the in vitro Culture of Banana Plantlets. Am. J. Food Technol. 2014; 9, 340-349.

Rahman, S., Biswas, N., Hassan, M.M., Ahmed, M.G., Mamun, A., Islam, M.R., Moniruzzaman, M., and Haque, M.E. (2013). Micropropagation of banana (Musa sp.) cv. Agnishwar by in vitro shoot tip culture. Int. Res. J. Biotechnol. 2013; 4, 83-88.

Yusnita, Y., Danial, E., and Hapsoro, D. (2015). In vitro shoot regeneration of Indonesian bananas (Musa spp.) cv. Ambon Kuning and Raja Bulu, plantlet acclimatization and field performance. Agrivita 2015; 37, 51-58.

Khatun, F., Hoque, M., Huq, H., Adil, M., Ashraf-Uz-Zaman, K., and Rabin, M.H. (2017). Effect of BAP and IBA on in vitro Regeneration of Local Banana Variety of Sabri. Biotechnol. J. Int. 2017; 18, 1-10.

Cruz-Rosero, N., Canchignia-Martínez, H., Morante-Carriel, J., Nieto-Rodríguez, E., Cruz-Rosero, E., Cabrera-Casanova, D., Izquierdo-Oviedo, H., Disotuar, R., González, M.C., and González, S.J. (2016). In vitro propagation of the Fiorito banana cultivar (Musa acuminata AA). Biotecnol Apl. 2016; 33, 4201-4204.

Karule, P., Dalvi, V., Kadu, A., Chaudhari, R., Subramaniam, V., and Patil, A. (2016). A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem. Afr. J. Biotechnol. 2016; 15, 401-407.

Venkatachalam, L., Sreedhar, R., and Bhagyalakshmi, N. (2007). Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers. Plant Growth Regul. 2007; 51, 193-205.

Bairu, M.W., Stirk, W.A., Doležal, K., and van Staden, J. (2008). The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tissue Organ Cult. 2008; 95, 373-379.

Makara, A., Rubaihayo, P., and Magambo, M. (2010). Carry-over effect of Thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions. Afr. J. Biotechnol. 2010; 9, 3079-3085.

Strosse, H., Andre, E., Sági, L., Swennen, R., and Panis, B. (2008). Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize. Plant Cell Tissue Organ Cult. 2008; 95, 321-332.

Youmbi, E., Ella, B., and Tomekpe, K. (2006). Effect of thidiazuron on in vitro proliferation capacities of some banana (Musa spp.) cultivars with weak multiplication potential. Mediterr. Agric. Sci. 2006; 19, 255-259.

Kanchanapoom, K., and Promsorn, N. (2012). Micropropagation and in vitro germplasm conservation of endangered Musa balbisiana ‘Kluai Hin’ (BBB group). Afr. J. Biotechnol. 2012; 11, 6464-6469.

Daungban, S., Pumisutapon, P., Topoonyanont, N., and Poonnoy, P. (2017). Effects of Explants Division by Cutting, Concentrations of TDZ and Number of Sub-culture Cycles on Propagation of ‘Kluai Hom Thong’Banana in a Temporary Immersion Bioreactor System. Thai J. Sci. Technol. 2017; 6, 89-99.

Arinaitwe, G., Rubaihayo, P., and Magambo, M. (2000). Proliferation rate effects of cytokinins on banana (Musa spp.) cultivars. Sci. Hortic. 2000; 86, 13-21.

Farahani, F., Aminpoor, H., Sheidai, M., Noormohammadi, Z., and Mazinani, M. (2008). An improved system for in vitro propagation of banana (Musa acuminata L.) cultivars. Asian J. Plant Sci. 2008; 7, 116-118.

Manjula, R., Jholgiker, P., Subbaiah, K.V., Prabhuling, G., Swamy, G., and Kumar, Y.L. (2014). Morphological abnormality among hardened shoots of banana cv. Rajapuri (AAB) after in vitro multiplication with TDZ and BAP from excised shoot tips. Int. J. Agri. Environ.Biotechnol. 2014; 7, 465-470.

Gutiérrez-Mora, A., González-Gutiérrez, A.G., Rodríguez-Garay, B., Ascencio-Cabral, A., and Li-Wei, L. (2012). Plant somatic embryogenesis: some useful considerations. In Embryogenesis, Dr. Ken-Ichi Sato ed. (InTech), pp. 2012; 229-248.

Roy, O.S., Bantawa, P., Ghosh, S.K., da Silva, J.A.T., Ghosh, P.D., and Mondal, T.K. (2010). Micropropagation and field performance of ‘Malbhog’ (Musa paradisiaca, AAB group): a popular banana cultivar with high keeping quality of north east India. Tree for. Sci. Biotechnol. 2010; 4, 52-58.

Hussein, N. (2012). Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro. Afr. J. Biotechnol. 2012; 11, 9001-9006.

Ngomuo, M., Mneney, E., and Ndakidemi, P. (2013). The effects of auxins and cytokinin on growth and development of (Musa sp.) var. “Yangambi” explants in tissue culture. Am. J. Plant Sci. 2013; 4, 2174-2180.

Safwat, G., Abdul-Rahman, F., and El Sharbasy, S. (2016). The effect of some antioxidants on blackening and growth of in vitro culture of banana (Musa spp. cv. Grand naine). Egypt. J. Genet. Cytol. 2016; 44, 47-59.

Gübbük, H., and Pekmezci, M. (2004). In vitro propagation of some new banana types (Musa spp.). Turk. J. Agric. For. 2004; 28, 355-361.

Bandita, D., and Bikram, P. (2017). Effects of plant growth hormones on shoot proliferation of Musa paradisiaca cv. Bantal. ‎Int. J. Plant Sci. 2017; 12, 135-138.

Muhammad, A., Rashid, H., Hussain, I., and Naqvi, S.S. (2007). Proliferation-rate effects of BAP and kinetin on banana (Musa spp. AAA Group) ‘Basrai’. HortScience 2007; 42, 1253-1255.

Martin, K.P., Zhang, C.-L., Slater, A., and Madassery, J. (2007). Control of shoot necrosis and plant death during micro-propagation of banana and plantains (Musa spp.). Plant Cell Tissue Organ Cult. 2007; 88, 51-59.

Ngomuo, M., Mneney, E., and Ndakidemi, P. (2014a). Control of lethal browning by using ascorbic acid on shoot tip cultures of a local Musa spp. (Banana) cv. Mzuzu in Tanzania. Afr. J. Biotechnol. 2014a; 13, 1721-1725.

Bairu, M.W., Stirk, W.A., and Van Staden, J. (2009). Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tissue Organ Cult. 2009; 98, 239-248.

Ahmad, I., Hussain, T., Ashraf, I., Nafees, M., Maryam, R.M., and Iqbal, M. (2013). Lethal effects of secondary metabolites on plant tissue culture. Am. Eurasian J. Agric. Environ. Sci. 2013; 13, 539-547.

Ozyigit, I.I. (2008). Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips. Afr. J. Biotechnol. 2008; 7, 1145-1150.

Tabiyeh, D.T., Bernard, F., Shacker, H., 2006. Investigation of glutathione, salicylic acid and GA3 on browning in Pistacia vera shoot tips cultures. Acta Hortic. 2006; 726, 201–204.

Krishna, H., Sairam, R., Singh, S., Patel, V., Sharma, R., Grover, M., Nain, L., and Sachdev, A. (2008). Mango explant browning: Effect of ontogenic age, mycorrhization and pre-treatments. Sci. Hortic. 2008; 118, 132-138.

Ssekiwoko, F., Talengera, D., Kiggundu, A., Namutebi, M., Karamura, E., and Kunert, K. (2014). In-vitro proliferation of Musa balbisiana improves with increased vitamin concentration and dark culturing. J. appl. biol. biotechnol. 2014; 2, 001-007.

Chikezie, U. (2012). Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips. ISABB. J. Biotech. Bioinform. 2012; 2, 11-17.

Ndakidemi, C.F., Mneney, E., and Ndakidemi, P.A. (2014). Effects of ascorbic acid in controlling lethal browning in in vitro culture of Brahylaena huillensis using nodal segments. Am. J. Plant Sci. 2014; 5, 187-191.

Ko, W., Su, C., Chen, C., and Chao, C. (2009). Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Cell Tissue Organ Cult. 2009; 96, 137-141.

Ngomuo, M., Mneney, E., and Ndakidemi, P.A. (2014b). The in vitro propagation techniques for producing banana using shoot tip cultures. Am. J. Plant Sci. 2014b; 5, 1614-1622.

North, J., Ndakidemi, P., and Laubscher, C. (2012). Effects of antioxidants, plant growth regulators and wounding on phenolic compound excretion during micropropagation of Strelitzia reginae. Int. J. Phys. Sci.2012; 7, 638-646.

Thomas, T.D. (2008). The role of activated charcoal in plant tissue culture. Biotechnol Adv. 2008; 26, 618-631.

Titov, S., Bhowmik, S.K., Mandal, A., Alam, M.S., and Uddin, S.N. (2006). Control of phenolic compound secretion and effect of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. Am. J. Biochem. Biotechnol. 2006; 2, 97-104.

Buah, J., Danso, E., Taah, K., Abole, E., Bediako, E., Asiedu, J., and Baidoo, R. (2010). The effects of different concentrations cytokinins on the in vitro multiplication of plantain (Musa spp.). Biotechnology 2010; 9, 343-347.

Agbadje, E.T.A.-E.F., Agbidinoukoun, A., Zandjanakou-Tachin, M., Cacaï, G.T.H., Houedjissin, S.S., and Ahanhanzo, C. (2017). Effect of drying plantain (Musa spp.) suckers prior to in vitro culture on reduction of lethal browning. Int. J. Agric. Pol. Res. 2017; 5, 192-200

Onuoha, C., Eze, C., Unamba, C., and UGOCHUKWU, C. (2011). In vitro prevention of browning in plantain culture. Rom. J. Biol. - plant biol. 2011; 56, 123-130.

Singh, H., Uma, S., Selvarajan, R., and Karihaloo, J. (2011). Micropropagation for production of quality banana planting material in Asia-Pacific. Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB) Asia-Pacific Association of Agricultural Research Institutions (APAARI) (New Delhi, India 2011.

Husin, N., Jalil, M., Othman, R.Y., and Khalid, N. (2014). Enhancement of regeneration efficiency in banana (Musa acuminata cv. Berangan) by using proline and glutamine. Sci. Hortic. 2014,168, 33-37.

Thomas, P., Swarna, G.K., Roy, P.K., and Patil, P. (2008b). Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine. Plant Cell Tissue Organ Cult. 2008b; 93, 55–63.

Odutayo, O., Oso, R., Akinyemi, B., and Amusa, N. (2004). Microbial contaminants of cultured Hibiscus cannabinus and Telfaria occidentalis tissues. Afr. J. Biotechnol. 2004; 3, 473-476.

Odutayo, O.I., Amusa, N.A., Okutade, O.O., and Ogunsanwo, Y. (2007). Sources of microbial contamination in tissue culture laboratories in southwestern Nigeria. Afr. J. Agric. Res. 2007; 2, 067-072.

Omamor, I., Asemota, A., Eke, C., and Eziashi, E. (2007). Fungal contaminants of the oil palm tissue culture in Nigerian institute for oil palm research (NIFOR). Afr. J. Agric. Res. 2007; 2, 534-537.

Msogoya, T., Kanyagha, H., Mutigitu, J., Kulebelwa, M., and Mamiro, D. (2012). Identification and management of microbial contaminants of banana in vitro cultures. J. Appl. Biosci. 2012; 55, 3987-3994.

Helaly, M.N., El-Metwally, M.A., El-Hoseiny, H., Omar, S.A., and El-Sheery, N.I. (2014). Effect of nanoparticles on biological contamination of'in vitro'cultures and organogenic regeneration of banana. Aust. J. Crop Sci. 2014; 8, 612-624.

Bradbury, J. (1987). Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods. Acta hortic. 1987; 225, 27 - 37.

Obuekwe, C., and Osagie, I. (1989). Morphological changes in infected wilt-resistant and wilt-susceptible oil palm progenies and hydrolytic enzyme activities associated with the Fusarium oxysporum f. sp. elaeidis pathogens. Oléagineux (Paris) 1989; 44, 393-402.

Ryan, R.P., Germaine, K., Franks, A., Ryan, D.J., and Dowling, D.N. (2008). Bacterial endophytes: recent developments and applications. FEMS Microbiol. Lett. 2008; 278, 1-9.

Thomas, P., and Reddy, K.M. (2013). Microscopic elucidation of abundant endophytic bacteria colonizing the cell wall–plasma membrane peri-space in the shoot-tip tissue of banana. AoB Plants 2013; 5, plt011.

Thomas, P., and Sekhar, A.C. (2014). Live cell imaging reveals extensive intracellular cytoplasmic colonization of banana by normally non-cultivable endophytic bacteria. AoB Plants 2014; 6, plu002.

Tyler, H.L., and Triplett, E.W. (2008). Plants as a habitat for beneficial and/or human pathogenic bacteria. Annu. Rev. Phytopathol. 2008;46, 53-73.

CIDES (1999). Micropropagation en entreprise - cahier de références techniques (CIDES) 1999.

Anicker, B., Thomas, P., Janakiram, T., Venugopalan, R., and Narayanappa, S.B. (2007). Influence of cytokinin levels on in vitro propagation of shy suckering chrysanthemum “Arka Swarna” and activation of endophytic bacteria. In vitro Cell Dev Biol Plant. 2007, 43, 614-622.

Thomas, P., Swarna, G.K., Patil, P., and Rawal, R.D. (2008a). Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue cultures. Plant Cell Tissue Organ Cult. 2008a; 93, 39-54.

Dangi, M., Gill, M., and Saini, H. (2009). Factors affecting in vitro shoot regeneration in banana cv. Grand Naine. Indian J. Plant Physiol. 2009; 14, 39-43.

Mng’omba, S.A., du Toit, E.S., Akinnifesi, F.K., and Venter, H.M. (2007). Effective preconditioning methods for in vitro propagation of Uapaca kirkiana Müell Arg. tree species. Afr. J. Biotechnol. 2007; 6, 1670-1676.

Azam, F.M.S., Islam, S., Rahmatullah M. and Zaman A. (2010). Clonal Propagation of Banana (Musa spp.) cultivar ‘BARI-1’ (AAA Genome, Sapientum Subgroup). Acta Hort, 2010; 537-544.

Silué, O.; Kouassi, K M., Koffi, K E., Kouakou, K. E. P., and Aké, S. (2017). Effect of adenine sulphate, casein hydrolysate and spermidine on in vitro shoot multiplication of two banana varieties (FHIA-21 and PITA-3). Afr. J. Biotechnol. 2017; 16 (46), 2152-2159.

Prabhath, G. P. W. A., and De Silva T. (2014). Micropropagation of M. balbisiana and cultivated banana variety “Ambun”, using immature male flowers. Asian J. Plant Sci. Res., 2014, 4 (4):54-61.


How to Cite
Agbadje, E. T. A. E., Agbidinoukoun, A., Zandjanakou-Tachin, M., Cacaï, G. T. H., & Ahanhanzo, C. (2021). Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review. European Journal of Biology and Biotechnology, 2(4), 1–8.