Scoparia dulcis (Linn) is a widespread herbal medicine; it bears an enormous number of pharmacological activities. The present study was undertaken to find out the chronic toxicity profile of oral administration of Scoparia dulcis ethanol leaf extract (SDELE) on the liver and the kidney of wistar rats. The animals were grouped into four and administered varying doses of SDELE (100 mg/kg, 200 mg/kg, 400 mg/kg body weight and 0.2 ml distilled water respectively) for a period of fourteen weeks (100 days). The acute toxicity, body weight, relative organ weight, hematological parameters, biochemical markers for liver and kidney damage were monitored and histopathology of the liver and kidney of the rat were carried out. The LD50 of SDELE was found to be 1131 mg/kg body weight. There was a significant (p<0.05) reduction in weight of the rat administered 400 mg/kg and 200 mg/kg when compared with the control though there was no significant difference (p>0.05) in the relative weight of the organs. There was also a significant increase (p<0.05) in the lymphocytes, serum level of aspartate amino transferase (ASP), alanine amino transferase (ALT), alkali phosphatase (ALP), total protein, A/G ratio, creatinine, urea, uric acid, total cholesterol, triacylglycerol, low density lipoprotein cholesterol and potassium ions while there was a significant decrease in HDL-cholesterol and sodium ions in the animal group administered 400 mg/kg body weight of the extract. Histopathology of the liver and kidney revealed haemorrhage and vascular congestion at 200 mg/kg doses and renal damage at 400 mg/kg body weight doses respectively. However, there was no significant difference (p>0.05) in any of the parameters studied in the group administered 100 mg/kg body weight dose when compared with the controlled group. Ethanol leaf extracts of Scoparia dulcis showed hepatotoxic and nephrotoxic tendencies and should be used with caution especially when employed in the treatment of chronic diseases
T, K. Hayashi, Y. Miwa, T. Taga, N. Morita, Antiviral agents of plant origin III. Scopadulin, a novel tetracyclic diterpene from Scoparia dulcis L. Chemical and Pharmaceutical Bulletin; 38, 1990: 945-947.
T. Hayashi., K. Okamura., Y. Tamada., A. Iida., T. Fujita., N. Morita, A new chemotype of Scoparia dulcis. Phytochemical Analysis, 33, 1993: 349—352.
M. Ahmed, H.A. Shikha, S.K. Sadhu, M.T. Rahman, B.K. Datta. Analgesic, diuretic, and anti-inflammatory principle from Scoparia dulcis. Pharmazie; 2001, 56(8):657–60.
J. Yisa, Phytochemical analysis and Antimicrobial activity of Scoparia dulcis and Nymphaea lotus. Australian Journal of Basic and Applied Sciences. Vol. 3(4), 2009: 3975-3979.
J.C. Tsai, W.H. Peng, T.H. Chiu, S.C. Huang, T.H. Huang, Z.R. Lai, Hepatoprotective effect of Scoparia dulcis on carbon tetrachloride induced acute liver injury in mice. American Journal of Chinese Medicine; 3, 2010:761-775.
A.I. Alli, J.O. Ehinmidu, and Y.K.E. Ibrahim, Preliminary Phytochemical Screening and Antimicrobial Activities of Some Medicinal Plants Used in Ebiraland. Bayero Journal of Pure and Applied Sciences, 2011, 4(1): 10 – 16.
J.B. Harborne, Phytochemical methods guide to modern Technique of Plant analysis. Edn 3, Chapman and Hall, London, 1998.
M. Kpodar, S. Lawson-Evi, P. Bakoma. et al., “Ethnopharmacological survey of plants used in the treatment of diabetes mellitus in south of Togo (Maritime Region),” Journal of Herbal Medicine, 2015, vol. 5, no. 3, pp. 147–152.
J. Vasquez, S.L. Jim´enez., I.C. G´omezet al., “Snakebites and ethnobotany in the Eastern region of Antioquia, Colombia—the traditional use of plants,” Journal of Ethnopharmacology, 2013, vol.146, no. 2, pp. 449–455.
M.O. Soladoye and O.O Oyesiku, “Taxonomy of Nigerian Medicinal Plants” in A Textbook of Medicinal Plants from Nigeria, T. Odubgemi, Ed., Akoka-Yaba Lagos: Lagos University Press, 2008, ch 11, pp 93-149.
S.E. Okhale, M.O. Amanabo, I.A. Jegede, H.O. Egharevba, I.W. Muazzam, O.F. Kunle, Phytochemical and Pharmacognostic Investigation of Antidiabetic Scoparia dulcis Linn Scrophulariaceae Whole Plant Grown in Nigeria. Researcher, 2010, 2 (16); 7-16.
A.I. Alli, J.O. Ehinmidu, and E.O. Igbadi, Evaluation of Antibacterial Properties, Acute Toxicity and Immuno-stimulatory Potential of Scoparia dulcis. Nigerian Journal of Biotechnology, 2013, Vol. 26: 21–25.
M.R. Mishra, A. Mishra, D.K. Pradhan, R.K. Behera, S. Jha, and A.K. Panda, Phytochemical screening of Scoparia dulcis L. (Scrophulariaceae) International Research Journal of Pharmaceutical Science, 2013, 4(1); 9-13.
M. Latha, L. Pari, Modulatory effect of Scoparia dulcis in oxidative stress induced lipid peroxidation in streptozotocin diabetic rats. Journal of Medicinal Food,2003, 6, 379–386.
M. Latha, L Pari, (2004). Efficacy of aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and polyol pathway in experimental diabetes. Brazilian Journal of Medical and Biological Research, 2004, 37, 577–586.
M. Latha L. Pari, S. Sitasawad, R. Bhonde, Insulin-secretagogue activity and cytoprotective role of the traditional antidiabetic plant Scoparia dulcis (Sweet Broom weed). Life Science, 2004, 75:2003-2014.
M. Latha, L. Pari, S. Sandhya, R. Bhonde. Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo. Journal of Biochemical and Molecular Toxicology,2004, 18(5), 261–272.
L. Pari, and M. Latha, Effect of Scoparia dulcis (Sweet Broom weed) plant extract on plasma antioxidants in streptozotocin-induced experimental diabetes in male albino Wistar rats. Die Pharmazie, 2004, 59, 557–560.
A.H. Zulfiker, M.R. Saha, S. Sarwar, L. Laizuman Nahar, K. Hamid, M.S. Sohel Rana, Hypoglycemic and in vitro antioxidant activity of ethanolic extracts of Ficus racemosa Linn. fruits. American Journal of Scientific and Industrial Research, 2010, 2(3): 391-400.
F.J. Evans, Constituents of Egyptian Euphorbiaceae. IX. Irritant and cytotoxic ingenane esters from Euphorbia paralias L. Experientia, 1980, 36:12067.
V.J. Navarro, J.R. Senior, Drug related hepatotoxicity. N England Journal of Medicine, 2006, 354:731-739.
A.Y. Onaolapo, O.J. Onaolapo, T.J. Mosaku, O.O. Akanji, O. Abiodun, A histological study of the hepatic and renal effects of subchronic low dose oral monosodium glutamate in Swiss albino mice. British Journal of Medical Research.2013,3(2):294-306.
D. Lorke, A new approach to practical acute toxicity testing. Archives of Toxicology, 1983, 54: pp 275–287.
Evenson M.E. “Spectrophotometric Technique”. In: Tietz Textbook of Clinical Chemistry, 3rd ed, CA Burtis, ER Ashwood, Eds. Philadelphia: WB Saunders Co., 1999, pp. 75-93.
J. Ochei and A. Kolhatkar, Textbook of Medical Laboratory Technology- Theory and Practice,3rd ed. 2008, Tata McGraw-Hill Publishing Company Limited, New Delhi: pp.180–182.
A.C. Adebajo, O.F. Ayoola, E.O. Iwalewa, A.A. Akindahunsi, N.O. Omisore, C.O. Adewunmi, Anti-trichomonal, biochemical and toxicological activities of methanolic extract and some carbazole alkaloids isolated from the leaves of Murraya koenigii growing in Nigeria. Phytomedicine. 2006, 13(4):246-254.
E.S. Orhue, M. Idu, J.E. Ataman, L.E. Ebite, Haematological and histopathological studies of Jatropha tanjorensis (J.L. Ellis and Soroja) leaves in rabbits. Asian Journal of Biological Sciences.2008, 84-89.
T.O. Elufioye, O.I. Alatise, F.A. Fakoya, J.M. Agbedahunsi, P.J. Houghton, Toxicity studies of Tithonia diversifolia A. Gray (Asteraceae) in rats. Journal of Ethnopharmacology,2009, 122 (2):410-415.
D.E. Cross, R.M. Mcdevitt, K. Hillman, T. Acamovic, The effects of herbs and associated oils on performance, dietary digestibility and gut microflora in chickens from 7 – 28 days of age. British Poultry Science. 48, pp 496–506.
S. Saeidnia, A.R. Gohari, N. Mokhber-Dezfuli, F. Kiuchi, Phytochemistry and medicinal properties of the genus Achille, DARU Journal of Pharmaceutical Sciences, 2011, 19 (3) 173-186.
M. Nematy, S.A.R. Mohajeri, S.A. Moghadam, M. Safarian, A Noroway, S.M.R. Parizadeh et al., Nutritional status in Intensive Care Unit patients: a perspective clinical, pilot study. Medical Journal of Nutrition and Metabolism, 2012, pp. 163–168.
T. Sanchez-Elsner, J.R. Ramirez, F. Rodriguez-Sanz, E. Varela, C. Bernabew, L.M. Botella,A cross talk between hypoxia and TGF-beta orchestrates erythropoietin gene regulation through SPI an SMADS. Journal of Molecular Biology,2004, 36: 9-24.
H.G. Olson, D. Bentton, K. Robinson, K. Thomas, A. Monro, A. et al., Concordance of the pharmaceutical in humans and in animals. Regulatory Toxicology and Pharmacology,2000, 32: 56-67.
L. Tedong, P.D.D. Dzeufiet, E.A. Dimo, S. N. Asongalem, J.F. Sokeng, Flejou, P. Callard, and P.K Kamtchouing, Acute and subchronic toxicity of Anacardium occidentale Linn (Anacardiaceae) leaves hexane extract in mice. African Journal of Traditional, Complementary and Alternative Medicine, 2007, 4 (2):140-147.
V.J. Navarro, J.R. Senior, Drug related hepatotoxicity. New England Journal of Medicine.2006, 354:731-739.
A. Kumar, N. Rai, N. Kumar, P. Gautam, J.S. Kumar, Mechanisms involved in hepatoprotection of different herbal products: a review. International Journal of Research in Pharmaceutical Science.2013, 4(2):112-117.
J. Ochei and A. Kolhatkar, Textbook of Medical Laboratory Technology- Theory and Practice,3rd ed. 2008, Tata McGraw-Hill Publishing Company Limited, New Delhi: pp.159–160.
A.F. ElGazar, A.A. Rezq, and H.M. Bukhari, Anti-hyperglycemic effect of saffron extract in alloxan-induced diabetic rats. European Journal of Biological Sciences, 2013, vol.5, pp.14-22.
M.A. Ferguson, V.S. Vaidya, J.V. Bonventre, Biomarkers of nephrotoxic acute kidney injury. Toxicology, 2008 245:182-193.
M.A. Perazella, Renal vulnerability to drug toxicity. Clinical Journal of American Society of Nephrology.2009, 4:1275-1283.
S. Karie, V. Launay-Vacher, G. Deray, C. Isnard-Bagnis, Drugs renal toxicity. Journal of Nephrology and Therapeutics;2010, 6(1):58-74.
A. Gnanamani, M. Sudha, G. Deepa, M. Sudha, K. Deivanai, S. Sadulla, 2008, Hematological and biochemical effects of polyphenolics in animal Full L e n g t h R e s e a r c h Paper models. Chemosphere, 72; 1321-1326.
J.T. Mukinda, F.K. Eagles, Acute and sub-chronic oral toxicity profile of the aqueous extract of Polygala fruticosa in female mice and rats. Journal of Ethnopharmacology, 2010, 128; 236-240.
E. Hecker, New toxic, irritant and cocarcinogenic diterpenoids esters from Euphorbiaceae and from Thymelaeaceae. Pure and Applied Chemistry; 1977, 49:1423-31.
M.D. Sayed, A. Rizk, F.M. Hammouda, M.M. El- Missiry, E.M. Williamson, F.J. Evans, Constituents of Egyptian Euphorbiaceae. IX. Irritant and cytotoxic ingenane esters from Euphorbia paralias L. Experientia, 1980, 36:12067.
A. Akintonwa, O. Awodele, G. Afolayan, H.A.B. Coker, Mutagenic screening of some commonly used medicinal plants. Journal of Ethnopharmacology. 2009, 125:461-470.
G.O. Oze, H.U. Nwanjo, G.O. Onyeze, Nephrotoxicity caused by the extract of A. boonei (De Wild) stem bark in guinea pigs. International Journal of Nutrition and Wellness; 2007, 3:2.
O. Awodele, V.O. Osunkalu, O.R. Akinde, J.A. Teixeira da Silva, W.O. Okunowo, E.C. Odogwu, et al. Modulatory roles of antioxidants against the aqueous stem bark extract of Alstonia boonei (Apocynaceae)-induced nephrotoxicity and testicular damage. International Journal of Biomedical and Pharmaceutical Science, 2010, 4(2):76-80.