Research Article

Effect of Extended Transit and Temperature Variations on EID50 Values of Thermostable NDVI-2 Vaccines

A. N. Egbuji
National Veterinary Research Institute, Nigeria
* Corresponding author
A. A. Chukwuedo
National Veterinary Research Institute, Nigeria
L. N. Shedua
National Veterinary Research Institute, Nigeria
J. K. Gyallak
National Veterinary Research Institute, Nigeria
L. N. Swomen
National Veterinary Research Institute, Nigeria
M. Muhammad
National Veterinary Research Institute, Nigeria
C. I. Nwosuh
National Veterinary Research Institute, Nigeria
L. U. Enurah
National Veterinary Research Institute, Nigeria
DOI icon 10.24018/ejbio.2020.1.5.76

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Submitted 2020-08-30
Published 2020-09-19

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Article Main Content

Temperature alterations due to poor cold keeping condition have consistently posed a challenge to vaccines dose EID50 titre value. The sensitivity of Newcastle disease vaccine to temperature variations due to poor cold storage condition in rural settings, led to the development of the NDVI-2 thermostable vaccine, which is known to exhibit a thermostability of 3 hours at 56 °C. In this study, three Newcastle disease vaccines (NDVI-2) with EID50 titre per dose values of log10 EID50 7.7, 7.6 and 6.75 respectively from vaccine production facility at the National Veterinary Research Institute (NVRI), Vom Nigeria, were sent for certification at African Union laboratory at Debre Zeit, Ethiopia under cold condition; using ice packs for cold chain maintenance. Tracking of the vaccine in the course of freight to the certifying laboratory revealed changes in the original transit route by the airline and delay by custom at the receiving country port. Thus, a 12 hours transit freight was delayed for over 7 days before arrival at the certifying laboratory. Though, the EID50 titre per dose values of these vaccines were originally well above recommended EID50 5.5 per dose value by log 2.2, log 2.1 and log 1.25 for NDVI-2 respectively. Comparing these vaccines EID50 values obtained from the certifying laboratory in relation to values initially obtained by the Quality Control Division of the producing Institute (NVRI), revealed  loss of EID50  titre per dose value by log101.2, and log10 0.8 among two of the certified vaccine batches; with one of the certified vaccines retaining its original EID50 titre per dose value. This result attest that NDVI-2 thermostable vaccine produced at NVRI, Vom Nigeria are robust and of high quality. Thus, the findings of this study have further demonstrated that temperature variations, prolonged vaccine transportation under poor cold chain can affect the EID50 per dose value of a wholesome, field fit vaccine irrespective of the vaccine thermostability.

Keywords: Transit Cold Condition Alterations, EID50 per Dose Values, Thermostable Newcastle Disease Vaccine

References

  1. OIE, (2013). Office of International Epizootics – Terrestrial Manual of Standards.
     Google Scholar
  2. B.S. Seal, D.J. King, H.S. Seller. “The avian response to Newcastle disease virus”, Dev. Comparative Immunology. Vol. 24, pp. 257-268, 2000.
     Google Scholar
  3. E.W. Aldous, J.K. Mynn, D.J. Alexander. “A molecular epidemiology study of avian paramyxovirus type 1 (Newcastle disease) isolate by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene”, Avian Pathology, Vol. 32, pp. 239-256, 2003.
     Google Scholar
  4. D. J. Alexander, and R.F .Jones. ” Newcastle disease, other paramyxovirus and pneumovirus infections”. In: Y.M. Saif (Ed) Diseases of poultry, Iowa State Press, pp. 63-92. 2003.
     Google Scholar
  5. M.H. Haque, M.T. Hossain, M.T.Islam, M.A. Zinnah, M.S.R. Khan, M.A. Islam.” Isolation and Detection of Newcastle disease virus from field outbreaks in Broiler and Layer chickens by Reverse Transcription-Polymerase chain reaction”. Journal. Veterinary Medicine. Vol. 8, pp. 87-92, 2010.
     Google Scholar
  6. N. Iran, M.S. Shah, F. Ismat, M. Habib, M. Iqbal, S.S. Hasnain, M. Rahman.” Heterologous expression, characterization and evaluation of the matrix protein from Newcastle disease virus as a target for antiviral therapies”. Applied. Microbiology and Biotechnology. Vol. 9, pp. 1691-701, 2013.
     Google Scholar
  7. L. Sa’idu, P.A., Abdu and E.L. Markus: ”Retrospective studies on Newcastle disease in vaccinated flocks in Zaria, Nigeria”. Student Veterinarian, Vol. 17, pp. 27-31, 1998.
     Google Scholar
  8. J.R .Lawal, S.M. Jajere, M. Mustapha, A.M. Bello, Y. Wakil, Y.A. Geidam, U.I .Ibrahim, I.A Gulani. “Prevalence of Newcastle Disease in Gombe, North-eastern Nigeria: A Ten-Year Retrospective Study (2004 –2013)”, British. Microbiology. Research. Journal, Vol. 6, pp. 367-375, 2015.
     Google Scholar
  9. T. Lurthu, Reetha, Johnson, J. Rajeswar. T. J. HariKrishnan, K. Sukumar, P. Srinivasan, J. John Kirubakaran. ” Studies on the effectiveness of oral pellet vaccine in improving egg production and egg quality in desk chicken”. Veterinary World, Vol. 9, pp. 900-903, 2016.
     Google Scholar
  10. R.P. Hanson (1972).” Newcastle disease. In: Diseases of Poultry 6th Ed. Edited by M.S. Hofstad Iowa State University Press.
     Google Scholar
  11. Anthony N. Egbuji, John O. Ibu, Ismaila A. Shittu, Dauda G. Bwala, Anne. S. Lohlum, Josephine Kiagama, Evelyn D. Dung, Uwanibe L.Enurah, Fatima B Mukaila, Dung S Lokason, Chinemerem. R Obene, Choji A Kwanga, Nicodemus M. Useh, Paul A Abdul, Usman Musa, John A. Okoye, Jude S. Rabo ” Immune Profiling of Grain Adapted I-2 (GAI-2) Pelleted Feed Vaccine in 6 Weeks Old Cockerels in Vom Nigeria”, Journal of Experimental Biology and Agricultural Sciences, Vol. 5, pp. 599-605, 2017.
     Google Scholar
  12. Zoth, S. Chimeno, E.Gómez, E. Carrillo, and A. Berinstein.” Locally produced mucosal IgG in chickens immunized with conventional vaccines for Newcastle disease virus”, Brazilian Journal of Medical and Biological Research, Vol. 41, pp. 318-323.2008 ISSN 0100-879X.
     Google Scholar
  13. OIE.” Office of International Epizootics – Terrestrial Manual of Standards”.2010.
     Google Scholar
  14. G. Karber. “50% end point calculation.” Archive für Experimentelle Pathologie Pharmakologie, Vol. 162: pp 480-483. 1931.
     Google Scholar
  15. A.N. Egbuji, A. Chukwuedo, L.N. Shedua, J.K. Gyallak, L.N. Swomen, N.C. Obene, U.K. Akachi, H.N. Chijioke, K.O. Idahor, Muhammad, M. and Enurah, L. U. “Overdose Administration of Thermostable Newcastle Disease Vaccines to Naïve Unvaccinated 6 Weeks Old Cockerels at NVRI, Vom Plateau state, Nigeria”. Journal of Advances in Microbiology, Vol. 20, pp. 29-34, 2020.
     Google Scholar
  16. Ideris, A.L. Ibrahim, P.E. Spradbrow, and C. Hung Seng” Development of Food Pellet Newcastle Disease Vaccine”. In: Copland, J.W., ed. Newcastle Disease in Poultry: A New Food Pellet Vaccine. Canberra, ACIAR, 20-23. Implication for diagnosis and control. Book of proceedings 29th November-1st December 1987.
     Google Scholar
  17. Z. Bensink and P.Spradbrow. ”Newcastle disease virus strain I-2- a prospective thermostable vaccine for use in developing countries”. Veterinary Microbiology,Vol. 68, pp. 131-139, 1999.
     Google Scholar
  18. W.H. Allan, J.E. Lancaster. and B. Toth. ”Newcastle disease vaccines-their production and use”, FAO Animal Production Series No. 10. FAO, Rome. 1978.
     Google Scholar
  19. Aini, A.L. Ibrahim and P.B. Spradbrow. ”Field trials of a food-based vaccine to protect village chickens against Newcastle disease”. Research in Veterinary Science, Vol. 49, pp. 216 – 219, 1990.
     Google Scholar
  20. L.J.E. Orajaka, D.F. Adene, B.M. Anene, and E.A.Onuoha. “Seroprevalence of Newcastle disease in local chickens from Southeast derived savannah zone of Nigeria”, Revue Elev. Med. Vet. Pays. Trop; No 52, Vol. 3-4, pp. 185-188,1999.
     Google Scholar
  21. M. Usman. ”Effects of vaccination of chickens against Newcastle diseases with thermostable V4 and La Sota vaccines“, MSc Thesis. Department of Veterinary Surgery and Medicine, Ahmadu Bello University, Zaria, Nigeria, 2002.
     Google Scholar
  22. R.G. Alders and P.B. Spradbrow. “Controlling Newcastle Disease in Village Chickens”: a field manual. Canberra, Australian Centre for International Agricultural Research. Monograph 82. 112pp. 2001a.
     Google Scholar