Research Article

Comparative Assessment of the Microbiological and Physicochemical Quality of a Laboratory Brewed ‘Burukutu’ and Commercialized Products Sold in Some Markets in Port Harcourt Nigeria

Francis Sopuruchukwu Ire
University of Port Harcourt, Nigeria
* Corresponding author
Precious Afoke Edio
University of Port Harcourt, Nigeria
Ndukwe Maduka
Wellspring University, Nigeria
DOI icon 10.24018/ejbio.2020.1.5.85

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Submitted 2020-09-13
Published 2020-10-08

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Article Main Content

Burukutu is an indigenous, brown coloured, cloudy, alcoholic beverage traditionally brewed using mainly sorghum grains. This study was aimed at the comparison of microbial quality, physicochemical properties, proximate composition and minerals content of laboratory brewed burukutu (LBB) and commercial products sold in some markets in Port Harcourt. Sixteen (16) samples of the commercialized product were evaluated from Elele (EM), Rumuokoro (RM) and Choba (CM) markets, Port Harcourt, Rivers State using Standard Methods while LBB was produced in our laboratory using Standard Procedures. Our result indicated an increase in aerobic mesophiles, total coliforms, faecal coliforms, fungi and lactic acid bacteria (LAB) counts during the early stages of the brewing process of laboratory produced burukutu but only LAB was detected in the final product. The overall microbial count of burukutu from the three markets revealed that CM>EM>RM although all the samples had higher microbial count compared with the LBB. Staphylococcus aureus, Escherichia coli, Lactobacillus sp., Lactobacillus sp., Aspergillus sp. and Saccharomyces cerevisiae were present during the brewing stages of burukutu. Enterobacter aerogenes and Mucor sp. were detected in the commercial samples purchased from the three markets. Lactobacillus fermentum and L. brevis had the highest and least frequency of occurrence in all the samples, respectively. The pH, titratable acidity, alcohol content and soluble solids of the fermenting LBB were within the range of 3.0-3.4, 0.6-0.97%, 3.0-4.7% and 0.98-0.99%, whereas in the commercialized samples, the equivalent values were 3.0-3.1, 0.00-0.10%, 2.1-4.0% and 0.65-0.82%, respectively. Moisture, crude fat, ash, crude protein and carbohydrate content of the commercialized samples were within the range of 29.1-31.2%, 10.8-14.2%, 3.8-4.85%, 2.9-5.7%, and 44.7-51.7% compared to the range of values obtained in the LBB (52.1%, 15.7%, 9.8%, 7.6% and 15.4%), respectively. The following minerals elements K, Ca, Na, Zn and Mg present in the LBB were higher than the values obtained in the commercialized alcoholic beverage. The results from this study, suggest that the quality of LBB is better than the commercialized products. Therefore, it is recommended that in order to improve the overall quality of commercially brewed burukutu for the benefit of everyone irrespective of social status, good manufacturing practices (GMP) and good hygienic practices (GHP) should be strictly adhered to by local producers and vendors.

Keywords: Sorghum, Brewing, Burukutu, Microbiological, Physicochemical, Food Quality and Safety

References

  1. E. C. Stephen, C. D. U. Nwokwu, and K. Bashir. (June 2017). Comparative physico-chemical analysis of locally brewed beer (burukutu) from corn, millet and sorghum. Am. J Sci. Tech. [Online]. 4(3). pp. 43-48. Available: http://www.aascit.org/journal/ajst.
     Google Scholar
  2. A. C. Ogbonna, C. I. Abuajah, and I. A. Umanah. (July 2016). Burukutu: healthy and superior indigenous African traditional opaque beverage. Am. J Adv. Food Sci. Tech. [Online]. 4(1). pp. 29-37. doi:10.7726/ajafst.2016.1004.
     Google Scholar
  3. A. B. Yusuf, B. H. Gulumbe, Z. M. Kalgo, B. Aliyu, and M. Haruna. (May 2020). Microorganisms associated with the production of burukutu (an alcoholic beverage) in Kebbi State, Nigeria. Equity J Sci. Tech. [Online]. 7(1). pp. 67-73. Available: www.equijost.com.
     Google Scholar
  4. A. Atter, K. Obiri-Danso, G. A. Anyebuno, and W. K. Amoa-Awua. (July 2017). Effect of fermentation on the chemical quality of burukutu a traditional beer in Ghana. Annals, Food Sci. Tech. [Online].18(3). pp. 349-354. Available: www.afst.valahia.ro.
     Google Scholar
  5. C. M. A. Iwegbue, A. L. Ojelum, and F. I. Bassey. (June 2014). A survey of metal profiles in some traditional alcoholic beverages in Nigeria. Food Sci. Nutri. [Online]. 2(6). pp. 724-733. doi:10.1002/fsn3. 163.
     Google Scholar
  6. E. Evera, A. Abdallah, S. He, Z. Shuang, W. Sainan, and H. Yu. (2019). Shelf life and nutritional quality of sorghum beer: potentials of phytogenic-based extracts. J Agric. Food Tech. [Online]. 9(2). pp. 1-14. Available: www.textroad.com.
     Google Scholar
  7. C. K. Egemba, and V. E. Etuk. (2007). A Kinetic Study of Burukutu Fermentation. J Engineer. App. Sci. [Online]. 2(7). pp. 1193-1198.
     Google Scholar
  8. S. T. Ogunbanwo, A. O. Adewara, and P. T. Fowoyo. (2013). Effect of fermentation by pure cultures of Lactobacillus fermentum 1 and Saccharomyces cerevisiae as starter cultures in the production of burukutu. New York Sci. J. [Online]. 6(1). pp. 73-81. Available: http://www.sciencepub.net/newyork.
     Google Scholar
  9. M. N. Alo, U. A. Eze, and N. E. Eda. (2012). Microbiological qualities of burukutu produced from a mixture of sorghum and millet. Am. J Food Nutri. [Online]. 2(4). pp. 96-102. doi:10.5251/ajfn.2012.2.4.96.102. Available: http://www.scihub.org/AJFN.
     Google Scholar
  10. O. O. Oladipo, A. B. Saanu, A. B. Juliet, and I. O. Adebola. (2018). Microbiological evaluation and antibiotic susceptibility pattern of bacteria associated with burukutu, a non-alcoholic beverage. J Food Res. Sci. [Online]. 7(1). pp. 1-7. doi: 10.3923/jfrs.2018.1.7.
     Google Scholar
  11. I. J. Sunday, and I. I. Aondover. (2013). Development of equations for estimating energy requirements in processing local alcoholic beverage (burukutu) in Nigeria. Int. J Engineer. Resear. Appli. [Online]. 3(4). pp. 648-654. Available: www.ijera.com.
     Google Scholar
  12. T. O. Ajiboye, G. A. Iliasu, O. B. Ojewuyi, A. T. Abdulazeez, A. O. Muhammed, and F. L. Kolawole. (May 2014). Sorghum-based alcoholic beverage, burukutu, perturbs the redox status of the liver of male rats. Food Sci. Nutri. [Online]. 2(5). pp. 591-596. doi: 10.1002/fsn3.139.
     Google Scholar
  13. O. O. Olaniyi, and J. B. Akinyele. (July 2019). Isolation of toxigenic Aspergillus flavus and evaluation of aflatoxins in burukutu, sorghum fermented beverage sold in Akure, Nigeria. J Food Safe Hyg. [Online]. 5(1). pp. 30-38. Available: http://jfsh.tums.ac.ir.
     Google Scholar
  14. C. G. Anaukwu, F. C. Nwangwu, O. I. Okafor, C. C. Ezemba, C. C. Orji, K. C. Agu, and E. J. Archibong. (2015). Microbiological analysis of burukutu beverage produced in southern part of Nigeria. Eur. J Exp. Biol. [Online]. 5(8). pp. 18-22. Available: www.pelagiaresearchlibrary.com.
     Google Scholar
  15. V. C. Eze, O. I. Eleke, and Y. S. Omeh. (2011). Microbiological and nutritional qualities of burukutu sold in mammy market Abakpa, Enugu State, Nigeria. Am. J Food Nutri. [Online]. 1(3). pp. 141-146. doi:10.5251/ajfn.2011.1.3.141.146, Available: http://www.scihub.org/AJFN.
     Google Scholar
  16. D. H. Larone. Medically important fungi, a guide to identification. Hagerston, Maryland, USA, London, Harper and Row. 1976, Vol. 2.
     Google Scholar
  17. D. Frey, R. J. Old-Field, and R. C. Bridjer. A colour atlas of pathogenic fungi. Wolf Medical Public Ltd. London. 1979.
     Google Scholar
  18. R. A. Samson, E. S. Hockstra, J. C. Frisvad, and D. Filtenburg D. Introduction to food borne fungi. Central bureau. Voor schimnel culture, Baarn Delft. Printed by Ponsen and Looyen. Wagening, the Netherlands. 1995, 4th edition.
     Google Scholar
  19. J. Dvorak, and M. Atanasek. Mycological diagnosis of animal dermatophytosis. Academic press 1969, pp. 213.
     Google Scholar
  20. Bergey’s Manual of Determinative Bacteriology. Holt, J.D. (Ed.), Williams Wilkins CO. Baltimore, 1994, 9th edition.
     Google Scholar
  21. M. Cheesbrough. District Laboratory Practice in Tropical Countries. Cambridge University Press, United Kingdom, 2005, 2nd edtion.
     Google Scholar
  22. J. C. de Man, M. Rogosa, M. E. Sharpe. (1960). A medium for the cultivation of lactobacilli. J Appli. Bacteriology. 23. pp. 130-135.
     Google Scholar
  23. AOAC (1990). Methods of Analysis of the Association of Official Analytical Chemists. Arlington: Association of Official Analytical Chemists, Incorporated. 15th edition. Vol. 2. pp. 739-750.
     Google Scholar
  24. AOAC (2005). Official Methods of Analysis. Association of Official Analytical, Chemists International, Maryland, USA. 18th edition.
     Google Scholar
  25. A. Atter, K. Obiri-Danso, and W. K. Amoa-Awua. (April 2014). Microbiological and chemical processes associated with the production of burukutu a traditional beer in Ghana. Int. Food Resear. J. [Online]. 21(5). pp. 1769-1776. Available: http://www.ifrj.upm.edu.my.
     Google Scholar
  26. M. L. Lefyedi, G. J. Marias, M. F. Dutton, and J. R. N. Taylor. (2005). The microbial contamination, toxicity and quality of turned and unturned outdoor floor malted sorghum. J. Institute Brewing. [Online]. 111(2). pp. 190-196.
     Google Scholar
  27. M. L. Lefyedi, and J. R. N. Taylor. (2007). Control of the growth of coliforms and moulds in sorghum malting by bacterial and yeast cultures. The Institute Brewing Distilling. [Online]. 113(2). pp. 123-129.
     Google Scholar
  28. M. Umar, I. B. Mohammed, I. M. Abdulkarim, G. Yusuf, A. A. Yaya, and G. Leo. (March 2016). Comparative studies on the prevalence of Salmonella species in two homemade fermented beverages (zobo and kunu-zaki) sold at Samaru, Zaria, Kaduna, Nigeria. Int. J Sci. Resear. Pub. [Online]. 6(3). pp. 428-435. Available: www.ijsrp.org.
     Google Scholar
  29. C. R. Falegan, and S. O. Akoja. (2014). Microbiological and physicochemical studies of two Nigerian fermented alcoholic drinks (palm wine and burukutu) in Ekiti state, Nigeria. Eur. J Food Sci. Tech. [Online]. 2(2). pp. 13-22. Available: www.eajournals.org.
     Google Scholar
  30. I. E. Mbaeyi-Nwaoha, and N. I. Egbuche. (August 2012). Microbiological evaluation of sachet water and street-vended yoghurt and zobo drinks sold in Nsukka metropolis. Int. J Bio. Chem. Sci. [Online]. 6(4). pp. 1703-1717. Doi: http://dx.doi.org/10.4314/ijbcs.v6i4.27. Available: http://ajol.info/index.php/ijbcs.
     Google Scholar
  31. D. Matoulková, E. Vontrobová, M. Brožová, and P. Kubizniaková. (2018). Microbiology of brewery production-bacteria of the order Enterobacterales. Kvasny Prum. [Online]. 64(4). pp. 161-166. DOI:10.18832/kp201824.
     Google Scholar
  32. O. M. Kolawole, R. M. O. Kayode, and B. Akinduyo. (March 2007). Proximate and microbial analyses of burukutu and pito produced in Ilorin Nigeria. Afri. J Biotech. [Online]. 6(5). pp. 587-590. Available: http://www.academicjournals.org/AJB.
     Google Scholar
  33. A. O. Adewara, and S. T. Ogunbanwo. (2013). Effects of processing variables on the production of burukutu, a Nigerian fermented beverage. Nature Sci. [Online]. 11(1). pp. 16-28. Available: http://www.sciencepub.net/nature.
     Google Scholar
  34. F. O. Oladeinde, E. I. Nwankwo, O. A. Moronkola, M. A. Amosu, and B. Farayola. (2002). Determination of indigenous and foreign alcoholic beverages’ levels in urine by quantitative spectroscopy. Afri. J Biomed. Resear. [Online]. 5. pp. 73-76.
     Google Scholar
  35. I. S. Eneji, A. A. Asan, and A. U. Itodo. (April 2017). Physicochemical and microbial analysis of locally fermented drinks (burukutu and pito) from cereals in north central Nigeria. FUW Trends Sci. Tech. J. [Online]. 2(1B). pp. 433-438. Available: www.ftstjournal.com.
     Google Scholar
  36. R. J. de Souza, J. F. Swain, L. J. Appel, and F. M. Sacks. (2008). Alternatives for macronutrient intake and chronic disease: a comparison of the OmniHeart diets with popular diets and with dietary recommendations. Am. J Clin. Nutr. [Online]. 88(1). pp. 1-11. Available: https://academic.oup.com/ajcn/article/88/1/1/4648889.
     Google Scholar
  37. J. Slavin, and J. Carlson. (2014). Carbohydrates. Adv. Nutri. [Online]. 5. pp. 760-761. doi:10.3945/an.114.006163. Available: https://academic.oup.com/advances/article/5/6/760/4616690.
     Google Scholar
  38. J. Wylie-Rosett, K. Aebersold, B. Conlon, C. R. Isasi, and N. W. Ostrovsky. (April 2013). Health effects of low-carbohydrate diets: where should new research go? Curr. Diabetes Reports. [Online]. 13(2). pp. 271-278. doi:10.1007/s11892-012-0357-5.
     Google Scholar
  39. I. F. Fadahunsi, S. T. Ogunbanwo, and A. O. Fawole. (2013). Microbiological and nutritional assessment of burukutu and pito (indigenously fermented alcoholic beverages in West Africa) during storage. Nature Sci. [Online]. 11(4). pp. 98-103. Available: http://www.sciencepub.net/nature.
     Google Scholar